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Test Code HEM14 Thrombin Time w/ Mixing Studies

Synonym

  • TTMS
  • TT/Mixing
  • TT with Mixing

Specimen Required

Preferred Container/Tube: 3.2% Citrate Blue Top - 2.7 mL
Specimen Volume: 2.7 mL
Acceptable Container/Tube: 3.2% Citrate Blue Top - 1.8 mL
Specimen Volume: 1.8 mL
Platelet poor plasma (PPP):
Specimen Volume: 2 - 1 mL aliquots platelet poor plasma (PPP)
Specimen Minimum Volume: 1 - 1 mL aliquot platelet poor plasma (PPP)

Specimen Collection Information

For Outreach, other than OSU Patient Service Centers: Samples must be processed and received frozen/must remain frozen in transit. 

For sending frozen plasma specimens, process immediately upon receipt. Centrifuge capped specimen tube in accordance with CLSI H21, at a speed and time required to consistently produce platelet-poor plasma (PPP = platelet count <10 K/uL). Transfer PPP into plastic aliquot tubes, cap and freeze. DO NOT transport until specimens are completely frozen. 

Specimen Stability Information

Ambient: 
     Whole blood: 1 hour
      Platelet poor plasma: 4 hours
Frozen: 
      Platelet poor plasma: PPP removed from the cells and frozen at -20°C or ideally -70°C.

Rejected Due To

  • Ambient WB specimens >1 hour old
  • Ambient PPP specimens >4 hours old
  • Clotted specimens
  • Underfilled or overfilled tube
  • Hemolysis

Testing Algorithm

If the TT is prolonged, then TT mixing studies will be performed at an additional charge.

Reflex Tests

Reporting Name Available Separately Always Reported
Thrombin Time Mixing Studies Yes No

Reference Values

13.0-20.0 sec

Method Description

TT: Mechanical clot detection of undiluted plasma in the presence of a predetermined quantity of thrombin, resulting in a fibrin clot.

TT Mixing Study: Thrombin Time is performed subsequent to a 1:1 mix with normal plasma pool and also in the presence of protamine sulfate (if indicated).

Performing Lab

Clinical Lab UH

Day(s) Performed

Monday through Sunday

Report Available

Same day/1 day

Reporting Name

TT With Mixing Studies

CPT Code Information

85670
85675 x2 (if appropriate)

LOINC Code Information

46717-5
33673-5 (if appropriate)
33525-7 (if appropriate)