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Test Code IMM91 Monoclonal Prot Immuno, Serum

Clinical Information

Serum Protein Electrophoresis (SPE) is used for screening patients with suspected monoclonal gammopathies, but it alone is not considered an adequate screen for monoclonal gammopathies. For suspected monoclonal gammopathies, serum free light chains are recommended to be ordered together with SPE. Fibrinogen will migrate as a distinct band in the beta-gamma fraction, so plasma samples are not acceptable. Radiographic agents may produce an uninterpretable pattern. Immunotyping of monoclonal (M-) proteins identifies the monoclonal immunoglobulin heavy chain type (gamma, alpha, mu, delta, or epsilon) and light chain type (kappa or lambda) in serum. If a monoclonal light-chain is detected in the absence of an associated monoclonal heavy-chain, immunofixation electrophoresis specific for delta and epsilon chains is performed. Immunofixation is not a quantitative assay. If a monoclonal protein is identified, a serum protein electrophoresis assay is required for quantifying the abnormality. 

Synonym

  • SERUM TP
  • PSE
  • QIMM
  • SIMFX

Profile Information

Reporting Name Available Separately Always Reported
Total Protein No Yes
Serum Protein Electrophoresis Yes Yes
Immunoglobulins Yes Yes
Serum Immunofixation Yes Yes

Specimen Required

Preferred Container/Tube: Gold SST Top
Acceptable Container/Tube: Gold SST Top
Specimen Volume: 3 mL
Specimen Minimum Volume: 1 mL

Specimen Stability Information

Refrigerated: 10 days
Frozen: 2 months

Rejected Due To

  • Hemolysis
  • Aged samples
  • Improperly stored samples
  • Plasma specimens

Reference Values

Female Male

Total Protein
0-30 days: 4.2-6.2 g/dL
31-182 days: 4.4-6.6 g/dL
183-365 days: 5.6-7.9 g/dL
1-18 years: 5.7-8.0 g/dL
19+ years: 6.4-8.3 g/dL

 

Albumin
3.5-5.0 g/dL

 

Alpha 1
0.2-0.4 g/dL

 

Alpha 2
0.5-1.0 g/dL

 

Beta
0.5-1.1 g/dL

 

Gamma
0.6-1.5 g/dL

 

IgA
0-30 days: 0-10 mg/dL
31-182 days: ≤42 mg/dL
183-365 days: 6-68 mg/dL
1-3 years: 15-111 mg/dL
4-6 years: 33-166 mg/dL
7-9 years: 28-180 mg/dL
10-12 years: 55-193 mg/dL
13-15 years: 62-241 mg/dL
16-18 years: 69-262 mg/dL
19-59 years: 66-433 mg/dL
60+ years: 90-410 mg/dL

 

IgG
0-30 days: 162-872 mg/dL
31-182 days: 311-664 mg/dL
183-365 days: 325-647 mg/dL
1-3 years: 451-1,202 mg/dL
4-6 years: 560-1,319 mg/dL
7-9 years: 485-1,473 mg/dL
10-12 years: 586-1,609 mg/dL
13-15 years: 749-1,640 mg/dL
16-18 years: 804-1,817 mg/dL
19-59 years: 600-1,714 mg/dL
60+ years: 600-1,560 mg/dL

 

IgM
0-29 days: 1-57 mg/dL
30-182 days: <128 mg/dL
183-365 days: <131 mg/dL
1-3 years: 35-184 mg/dL
4-6 years: 42-184 mg/dL
7-9 years: 30-165 mg/dL
10-12 years: 42-211 mg/dL
13-15 years: 34-225 mg/dL
16-18 years: 45-224 mg/dL
19-59 years: 45-281 mg/dL
60+ years: 30-360 mg/dL

 

Monoclonal 1
≤0.0 mg/dL

 

Monoclonal 2
≤0.0 mg/dL

 

Monoclonal 3
≤0.0 mg/dL

 

Monoclonal 4
≤0.0 mg/dL

Total Protein
0-30 days: 4.1-6.3 g/dL
31-182 days: 4.7-6.7 g/dL
183-365 days: 5.5-7.0 g/dL
1-18 years: 5.7-8.0 g/dL
19+ years: 6.4-8.3 g/dL

 

Albumin
3.5-5.0 g/dL

 

Alpha 1
0.2-0.4 g/dL

 

Alpha 2
0.5-1.0 g/dL

 

Beta
0.5-1.1 g/dL

 

Gamma
0.6-1.5 g/dL

 

IgA
0-30 days: ≤11 mg/dL
31-182 days: ≤40 mg/dL
183-365 days: 1-82 mg/dL
1-3 years: 9-137 mg/dL
4-6 years: 44-187 mg/dL
7-9 years: 58-204 mg/dL
10-12 years: 46-218 mg/dL
13-15 years: 29-251 mg/dL
16-18 years: 68-259 mg/dL
19-59 years: 66-433 mg/dL
60+ years: 90-410 mg/dL

 

IgG
0-30 days: 197-833 mg/dL
31-182 days: 140-533 mg/dL
183-365 days: 130-823 mg/dL
1-3 years: 413-1,112 mg/dL
4-6 years: 468-1,328 mg/dL
7-9 years: 582-1,441 mg/dL
10-12 years: 685-1,620 mg/dL
13-15 years: 590-1,600 mg/dL
16-18 years: 522-1,703 mg/dL
19-59 years: 600-1,714 mg/dL
60+ years: 600-1,560 mg/dL

 

IgM
0-29 days: 0-65 mg/dL
30-182 days: 6-84 mg/dL
183-365 days: 15-117 mg/dL
1-3 years: 30-146 mg/dL
4-6 years: 31-151 mg/dL
7-9 years: 21-140 mg/dL
10-12 years: 27-151 mg/dL
13-15 years: 26-184 mg/dL
16-18 years: 28-179 mg/dL
19-59 years: 45-281 mg/dL
60+ years: 30-360 mg/dL

 

Monoclonal 1
≤0.0 mg/dL

 

Monoclonal 2
≤0.0 mg/dL

 

Monoclonal 3
≤0.0 mg/dL

 

Monoclonal 4
≤0.0 mg/dL

Method Description

Capillary electrophoresis

Performing Lab

Clinical Lab UH

Day(s) Performed

Monday through Friday (excludes OSU holidays)

Report Available

3 to 5 days

Reporting Name

Serum Total Protein
Protein Electrophoresis
Immunoglobulins IgG IgA IgM
Immunofixation, Serum

CPT Code Information

84165
82784 x3
86334

LOINC Code Information

2885-2
1751-7
2865-4
2868-8
2871-2
2874-6
33358-3 x4