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Test Code LAB523 Estradiol, Enhanced

Clinical Information

The drug Fulvestrant (FASLODEX®) may cause falsely elevated estradiol results. For patients being treated with Fulvestrant, an alternate method that is not expected to show cross-reactivity to Fulvestrant, such as liquid chromatography-mass spectrometry (LC-MS), should be used. For assays employing antibodies, the possibility exists for interference by human anti-animal antibodies (i.e., heterophile antibodies) in the patient sample. Patients who have been regularly exposed to animals or have received immunotherapy or diagnostic procedures utilizing immunoglobulins or immunoglobulin fragments may produce antibodies, (eg, HAMA), that interfere with immunoassays. This may falsely elevate or falsely decrease the results.

Synonym

  • EE2B

Specimen Required

Preferred Container/Tube: Gold Top
Acceptable Container/Tube: Gold Top
Specimen Volume: 3 mL
Specimen Minimum Volume: 1 mL

Specimen Stability Information

Ambient: 20 hours
Refrigerated: 5 days

Rejected Due To

  • Gross hemolysis

Interpretation

Male: 19+ years: <11.8-39.8 

 

Menstruating Females: 

  • Follicular phase: 19.5-144.2 
  • Midcycle peak: 63.9-356.7 
  • Luteal phase: 55.8-214.2 

Post-menopausal: <11.8-32.2 

 

This test is not recommended for patients receiving Fulvestrant (Falsodex) due to possible false elevations. EE2 levels vary widely throughout the menstrual cycle.

Method Description

Competitive assay format. The endogenous estradiol contained in a sample is released from its binding proteins by a releasing agent. Then, a sheep monoclonal anti-estradiol antibody labeled with acridinium ester is added to bind available estradiol. Finally, an estradiol derivative capture solid phase is added to the reaction to compete with estradiol for the binding of the acridinium-labeled antibody.  After washing, acid and base are dispensed to initiate the chemiluminescent reaction.

Performing Lab

Clinical Lab UH

Day(s) Performed

Monday through Sunday

Report Available

Same day/1 day

Reporting Name

Estradiol, Enhanced

CPT Code Information

82670

LOINC Code Information

2243-4