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HelpTest Code INPT454 Rabies Antibody Endpoint
Additional Codes
YRAB
Specimen Required
Specimen Type: Serum
Collection Container/Tube:
Preferred: Red top
Acceptable: Serum gel
Specimen Volume: 1 mL
Collection Instructions:
1. Centrifuge and aliquot 1 mL of serum into a plastic vial.
2. Send refrigerate.
Note: Collection date is required.
Method Name
Serum Neutralization Fluorescent Antibody
Reporting Name
Rabies RFFIT Endpoint, SSpecimen Type
SerumSpecimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Refrigerated (preferred) | 14 days |
| Frozen | 60 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Bacterial contamination | Reject |
Reference Values
Reportable range is 0.1 IU/mL to 15.0 IU/mL
Less than 0.1 IU/mL: Below detection limit
Interpretation
Quantitative results. For those who want to know their exact titer between the reportable range.
In humans, a result of 0.5 IU/mL or higher is considered an acceptable response to rabies vaccination according to the World Health Organization (WHO) guidelines; see WHO and Advisory Committee on Immunization Practices documents for additional guidance.
Method Description
In RFFIT, patient serum (the non-cellular portion of blood) is first diluted fivefold (1 part serum in 4 parts diluent). Further (serial fivefold) dilutions are performed, each of which contain less and less patient serum. These serum dilutions are mixed with a standard amount of live rabies virus and incubated. Whatever RVNA is present will neutralize the virus. Next, some tissue culture cells are added and the serum/virus/cells are incubated together. Whatever rabies virus is left (ie, that which has not been neutralized by the antibody in your serum), will infect the cells and this can be seen under the microscope through the use of specific staining. Calculation of the endpoint titer is made from the percent of virus infected cells observed on the slide.
Day(s) Performed
Monday, Wednesday, Thursday
Report Available
16 to 30 daysPerforming Laboratory
RFFIT/K-State Rabies Laboratory Manhattan/K-State Innovation CenterCPT Code Information
86382
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| FRFIT | Rabies RFFIT Endpoint, S | 6524-3 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| Z2071 | Rabies Titer-Response | 6524-3 |
Clinical Information
RFFIT stands for Rapid Fluorescent Foci Inhibition Test. It is a serum neutralization (inhibition) test, which means it measures the ability of rabies specific antibodies to neutralize rabies virus and prevent the virus from infecting cells. The antibodies are called rabies virus neutralizing antibodies (RVNA).