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Test Code INPT454 Rabies Antibody Endpoint

Additional Codes

YRAB


Specimen Required


Specimen Type: Serum

Container/Tube: Red

Specimen Volume: 2 mL

Collection Instructions: Draw blood in a plain red-top tube(s), Spin down and send 2 mL of serum refrigerated in a plastic vial.

 

Note:  1. Serum gel tube is acceptable, but must be poured off into plastic vial.

          2. Collection date is required.


Method Name

Serum Neutralization Fluorescent Antibody

Reporting Name

Rabies RFFIT Endpoint, S

Specimen Type

Serum

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 14 days
  Frozen  60 days

Reject Due To

Hemolysis Mild OK; Gross reject
Lipemia Mild OK; Gross reject
Icterus NA
Other NA

Reference Values

Reportable range is 0.1 to 15.0 IU/mL

Less than 0.1 IU/mL: Below detection limit

 

In humans a results of 0.5 IU/mL or higher is considered an acceptable response to rabies vaccination according to the World Health Organization (WHO) guidelines; see WHO and Advisory Committee on Immunization Practices documents for additional guidance.

 

Interpretation

Quantitative results. For those who want to know their exact titer between the reportable range.

 

RFFIT stands for Rapid Fluorescent Foci Inhibition Test. It is a serum neutralization (inhibition) test, which means it measures the ability of rabies specific antibodies to neutralize rabies virus and prevent the virus from infecting cells. The antibodies are called rabies virus neutralizing antibodies (RVNA).

Method Description

In RFFIT, your serum (the non-cellular portion of your blood) is first diluted fivefold (1part serum in 4 parts diluent). Further (serial fivefold) dilutions are performed, each of which contain less and less of your serum. These serum dilutions are mixed with a standard amount of live rabies virus and incubated. Whatever RVNA is present will neutralize the virus. Next, some tissue culture cells are added and the serum/virus/cells are incubated together. Whatever rabies virus is left (i.e., that which has not been neutralized by the antibody in your serum), will infect the cells and this can be seen under the microscope through the use of specific staining. Calculation of the endpoint titer is made from the percent of virus infected cells observed on the slide.

Day(s) Performed

Monday, Wednesday, Thursday

Report Available

21 to 35 days

Performing Laboratory

RFFIT/K-State Rabies Laboratory Manhattan/K-State Innovation Center

CPT Code Information

86382

LOINC Code Information

Test ID Test Order Name Order LOINC Value
FRFIT Rabies RFFIT Endpoint, S 6524-3

 

Result ID Test Result Name Result LOINC Value
Z2071 Rabies Titer-Response 6524-3