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HelpTest Code LAB39 Vancomycin Level, Trough (Pre Drug Level)
Clinical Information
Because the concentration of vancomycin fluctuates considerably depending on the time from last dose, interpretation of the clinical significance of the vancomycin concentration must take into consideration the timing of the blood specimen.
Synonym
- VANCTR
Specimen Required
Preferred Container/Tube: Mint Green Top
Acceptable Container/Tube: Mint Green Top, Red Top
Specimen Volume: 3 mL
Specimen Minimum Volume: 1 mL
Specimen Stability Information
Ambient: 4 hours
Refrigerated: 5 days
Specimen Collection Information
Processing instructions: Draw 30 min - 1 hour PRIOR to dose.
Rejected Due To
- Gross hemolysis
- Gross icterus
- Quantity not sufficient
- Quality not acceptable
Reference Values
Therapeutic Range: 10.0-20.0 mcg/mL
Method Description
Serum or plasma is mixed with Reagent 1, which contains vancomycin labeled with the enzyme glucose-6-phosphate dehydrogenase (G6PDH). Subsequently, Reagent 2, which contains antibodies to vancomycin and the coenzyme nicotinamide adenine dinucleotide (NAD), is added. Vancomycin in the sample and vancomycin-labeled G6PDH compete for antibody binding sites. Enzyme activity decreases upon binding to the antibody, so the vancomycin concentration in the sample can be measured in terms of enzyme activity. Active enzyme converts oxidized NAD to NADH, resulting in an absorbance change that is measured spectrophotometrically.
Performing Lab
Clinical Lab UH
Clinical Lab University East
Day(s) Performed
Monday through Sunday
Report Available
Same day/1 day
Reporting Name
Vancomycin Level, Trough (Pre Drug Level)
CPT Code Information
80202
LOINC Code Information
4092-3