Test Code CHEM50 ALP ALT AST LD GGT BILI
Synonym
- ENZ4GB
Profile Information
Reporting Name | Available Separately | Always Reported |
---|---|---|
Alkaline Phosphatase | Yes | Yes |
ALT | Yes | Yes |
AST | Yes | Yes |
LD Total | Yes | Yes |
GGT | Yes | Yes |
Bilirubin Total | Yes | Yes |
Bilirubin Direct | Yes | Yes |
Specimen Required
Preferred Container/Tube: Mint Green Top
Acceptable Container/Tube: Mint Green Top, Gold SST Top
Specimen Volume: 3 mL
Specimen Minimum Volume: 2 mL
Specimen Stability Information
Ambient: 6 hours
Specimen Collection Information
Avoid Hemolysis. Serum should be separated from the cells within two hours after collection.
Outpatient shipping instructions: Protect from light.
Rejection Due To
- Gross hemolysis
- Gross icterus
- Quantity not sufficient
Reference Values
FEMALE | MALE |
---|---|
ALP
LD
GGT
TBIL
DBIL |
ALP
ALT
AST
LD
GGT
TBIL
DBIL |
Cautions
LD and AST (and to a lesser extent ALP and ALT) may be significantly falsely elevated in the presence of hemolysis. Falsely low ALT results may occur in patients treated with sulfasalazine.
Samples with glycine, monoethanolamine, and high concentrations of phosphate and chloride may interfere with ALP measurements. Total and direct bilirubin results are falsely decreased in the presence of hemolysis. In some cases of monoclonal gammopathies (especially Waldenstrom's macroglobulinemia with very high concentrations of IgM), total and direct bilirubin results may be unreliable. Total and direct bilirubin results may be falsely low in the presence of high concentrations of N-acetyl-p-benzoquinone imine (metabolite of Acetaminophen).
Method Description
ALP
Photometric rate with p-nitro-phenylphosphate (pNPP) in the presence of 2-amino-2-methyl-1-propanol (AMP) at pH 10.4.
ALT
Photometric rate with alanine to α-oxoglutarate to form pyruvate and glutamate. This assay does not contain pyridoxyl-5-phosphate.
AST
Photometric rate with aspartate and α-oxoglutarate to form pyruvate and glutamate. This assay does not contain pyridoxyl-5-phosphate.
LD
Photometric rate
GGT
Photometric rate
DBIL
Photometric with diazonium salt, 3,5-dichloroaniline (DPD).
TBIL
Photometric with 3,5-dichlorophenyldiazonium tetrafluoroborate (DPD), and caffeine and a surfactant as accelerators.
Performing Lab
Clinical Lab UH
Clinical Lab University East
Clinical Lab Martha Morehouse
Clinical Lab Spielman
Clinical Lab West Campus
Day(s) Performed
Monday through Sunday
Report Available
Same day/1 day
Reporting Name
ALP ALT AST LD GGT BILI
CPT Code Information
84075
83615
84450
84460
82977
82247
82248
LOINC Code Information
6768-6
1742-6
1920-8
14804-9
2324-2
1975-2
1968-7