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Test Code DAT Direct Antiglobulin Test (DAT)

Clinical Information

Demonstrating in vivo coating of red blood cells with IgG or the complement component C3d in the following:

- Autoimmune hemolytic anemia
- Hemolytic transfusion reactions
- Drug-induced hemolytic anemia

Synonym

  • DATO
  • DAT 

Specimen Required

Preferred Container/Tube: Lavender Top
Acceptable Container/Tube: Lavender Top
Specimen Volume: 6 mL in 2 Lavender Top tubes each containing 3 mL
Specimen Minimum Volume: 1 mL in 1 Lavender Top tube

Specimen Collection Information

Specimens must arrive within 48 hours of collection.

Specimen Stability Information

Ambient: 24 hours
Refrigerated: 2 days

Rejected Due To

  • Frozen
  • Gross hemolysis
  • Sent on heat/ice
  • Less than minimum volume
  • Missing collector information
  • Missing patient identification

Reflex Tests

Direct Antiglobulin Test, IgG
Direct Antiglobulin Test, C3b/d
Antibody Elution testing

Reference Values

Negative

Interpretation

Negative: No IgG or complement detected on the surface of the red cell.
Positive: IgG and/or complement detected on the surface of the red cell.

Monospecific direct antiglobulin testing will be performed.

Cautions

Autoimmune hemolytic anemia can occur with a negative direct antiglobulin test. 

The direct antiglobulin test can be positive in some apparently healthy individuals.

Method Description

Hemagglutination

Performing Lab

Blood Bank University Hospital

Day(s) Performed

Monday through Sunday

Report Available

24 hours

Reporting Name

Direct Antiglobulin

CPT Code Information

86880

LOINC Code Information

54410-6