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Test Code INPT239 Baby Type and DAT (Direct Antiglobulin Test)

Clinical Information

Evaluation for hemolytic disease of the newborn (HDN) in infants of mothers with Group O blood, Rh-negative blood, or alloantibodies to non-ABO or Rh antigens; and determination of the mother's candidacy for Rh immune globulin therapy.

Weak D testing will be performed on all Rh-negative babies.

This test is not useful for monitoring the efficacy of Rh-immune globulin administration.

Synonym

  • HEELS
  • Heelstick Evaluation

Profile Information

ABO/Rh(D)
Direct Antiglobulin Test, Polyspecific

Specimen Required

Preferred Container/Tube: Lavender Top
Acceptable Container/Tube: Lavender Top, Microtainer EDTA
Specimen Volume: 1 mL in 2 tubes each containing 500 µL
Specimen Minimum Volume: 500 µL in 1 tube

Specimen Collection Information

Specimens must arrive within 48 hours of collection.

Specimen Stability Information

Ambient: 24 hours
Refrigerated: 2 days

Rejected Due To

  • Frozen
  • Gross hemolysis
  • Sent on heat/ice
  • Less than minimum volume
  • Missing collector information
  • Missing patient identification

Reference Values

DAT: Negative

Interpretation

ABO/Rh(D):
Standard ABO/Rh(D) type will be reported. Routine ABO Types include: A, B, O, AB.

Routine Rh(D) types include: Negative and Positive.

DAT, Polyspecific:
Negative: No IgG or complement detected on the surface of the red cell.
Positive: IgG and/or complement detected on the surface of the red cell. Monospecific direct antiglobulin testing will be performed.
 

Reflex Testing

Direct Antiglobulin Test, IgG
Direct Antiglobulin Test, C3b/d
Antibody Elution testing

Method Description

Hemagglutination

Performing Lab

Blood Bank University Hospital

Day(s) Performed

Monday through Sunday

Report Available

12 hours

Reporting Name

Heelstick Eval

CPT Code Information

86900
86901
86880