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HelpTest Code LAB30 Phenobarbital Level, Trough (Pre Drug Level)
Synonym
- PHNO
Specimen Required
Preferred Container/Tube: Mint Green Top
Acceptable Container/Tube: Mint Green Top, Red Top
Specimen Volume: 3 mL
Specimen Minimum Volume: 1 mL
Specimen Stability Information
Ambient: 4 hours
Refrigerated: 30 days
Specimen Collection Information
Outreach Clients: Refrigerate serum as soon as possible after separation from the cells.
Processing instructions: Draw 1 hour prior to dose. Specify draw time on requisition.
Rejected Due To
- Gross hemolysis
- Gross icterus
- Quantity not sufficient
- Quality not acceptable
Reference Values
Therapeutic Range: 15.0-40.0 mcg/mL
Method Description
The assay is based on competition between drug in the sample and drug labeled with the enzyme glucose-6-phosphate dehydrogenase (G6PDH) for antibody binding sites. Enzyme activity decreases upon binding to the antibody, so the drug concentration in the sample can be measured in terms of enzyme activity. Active enzyme converts nicotinamide adenine dinucleotide (NAD) to NADH, resulting in an absorbance change that is measured spectrophotometrically.
Performing Lab
Clinical Lab UH
Day(s) Performed
Monday through Sunday
Report Available
Same day/1 day
Reporting Name
Phenobarbital Level, Trough (Pre Drug Level)
CPT Code Information
80184
LOINC Code Information
3948-7