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Test Code LAB306 Factor VIII Activity

Clinical Information

Detection of specific coagulation factor VIII deficiency.

Synonym

  • FA8
  • Factor 8 Activity
  • Factor VIII Clotting
  • Anti-Hemophilic Factor

Specimen Required

Preferred Container/Tube: 3.2% Citrate Blue Top - 2.7 mL
Specimen Volume: 2.7 mL
Acceptable Container/Tube: 3.2% Citrate Blue Top - 1.8 mL
Specimen Volume: 1.8 mL
Platelet poor plasma (PPP):
Specimen Volume: 2 - 1 mL aliquots platelet poor plasma (PPP)
Specimen Minimum Volume: 1 - 1 mL aliquot platelet poor plasma (PPP)

Specimen Collection Information

For Outreach, other than OSU Patient Service Centers: Samples must be processed and received frozen/must remain frozen in transit. 

For sending frozen plasma specimens, process immediately upon receipt. Centrifuge capped specimen tube in accordance with CLSI H21, at a speed and time required to consistently produce platelet-poor plasma (PPP = platelet count <10 K/uL). Transfer PPP into plastic aliquot tubes, cap and freeze. DO NOT transport until specimens are completely frozen.

Specimen Stability Information

Ambient: 
     Whole blood: 4 hours
      Platelet poor plasma: 4 hours
Frozen: 
      Platelet poor plasma: PPP removed from the cells and frozen at -20°C or ideally -70°C.

Rejected Due To

  • Ambient specimens >4 hours old
  • Clotted specimens
  • Underfilled or overfilled tube
  • Hemolysis

Reference Values

0-4 days: 50-178 % Activity
5-29 days: 50-154 % Activity
30-89 days: 50-157 % Activity
90-179 days: 50-125 % Activity
180-365 days: 50-109 % Activity
1-5 years: 59-142 % Activity
6-10 years: 58-132 % Activity
11-16 years: 53-131 % Activity
17+ years: 50-200 % Activity

Interpretation

<1%: severe hemophilia
1- <5%: moderate hemophilia
5- <40%: mild hemophilia

Cautions

Interpretation of results may be limited if patient is receiving anticoagulant therapy.

Method Description

1 stage clotting assay

Performing Lab

Clinical Lab UH

Day(s) Performed

Monday through Friday (excludes OSU holidays)

Report Available

Same day/1 day

Reporting Name

Factor VIII Activity

CPT Code Information

85240

LOINC Code Information

3209-4