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Test Code LAB38 Tobramycin Level, Trough (Pre Drug Level)

Synonym

  • TOBTR

Specimen Required

Preferred Container/Tube: Mint Green Top
Acceptable Container/Tube: Mint Green Top, Red Top
Specimen Volume: 3 mL
Specimen Minimum Volume: 1 mL

Specimen Stability Information

Ambient: 4 hours
Refrigerated: 5 days

Specimen Collection Information

Processing instructions: Draw 30 min - 1 hour PRIOR to dose.

Rejected Due To

  • Gross hemolysis
  • Gross icterus
  • Quantity not sufficient
  • Quality not acceptable

Reference Values

Therapeutic Range: <1.0 mcg/mL

Method Description

This assay is based on competition for antibody binding sites between drug in the sample and drug labeled with the enzyme glucose-6-phosphate dehydrogenase (G6PDH). Enzyme activity decreases upon binding to the antibody, so the drug concentration in the sample can be measured in terms of enzyme activity. Active enzyme converts oxidized nicotinamide adenine dinucleotide (NAD) to NADH, resulting in an absorbance change that is measured spectrophotometrically.

Performing Lab

Clinical Lab UH

Day(s) Performed

Monday through Sunday

Report Available

Same day/1 day

Reporting Name

Tobramycin Level, Trough (Pre Drug Level)

CPT Code Information

80200

LOINC Code Information

4059-2