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Test Code LABBTFXM T&B Cell Flow Crossmatch

Synonym

  • BTFXM
  • B&T cell flow crossmatch

Specimen Required

Specimen Source: Donor
Preferred Container/Tube: ACD
Acceptable Container/Tube: ACD
Specimen Volume: 6 mL
Specimen Minimum Volume: 4 mL

 

Specimen Source: Recipient
Preferred Container/Tube: Red Top
Acceptable Container/Tube: Red Top, Serum Aliquot, Gold Top SST
Specimen Volume: 6 mL
Specimen Minimum Volume: 2 mL

Specimen Collection Information

Collect 3 ACD yellow top tubes from transplant donor. Donor specimen must be delivered within one day of collection.

 

Communicate STAT requests with lab and/or on-call staff.

Specimen Stability Information

Ambient: 3 days
Refrigerated: 3 days (lymph node and spleen only)

Rejected Due To

  • Hemolysis
  • Clotted specimens
  • Frozen specimens
  • Quantity not sufficient
  • Collection date greater than 7 days prior to receipt
  • Insufficient WBC count for DNA extraction
  • Inadequate lymph nodes from donor specimen
  • Donor specimen > 72 hours old

Method Description

Flow cytometric crossmatch, B- and T-cell

Performing Lab

Tissue Typing

Day(s) Performed

Monday through Friday (Stat requests 24/7)

Report Available

Deceased donor: 8 hours
Living donor: 2 to 5 days

Reporting Name

B & T Cell Flow Crossmatch

CPT Code Information

86825 x2