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Test Code LABGP1BM Von Willebrand Factor GP1bM Activity

Clinical Information

Aid with the diagnosis of Von Willebrand disease.

Synonym

GP1bM

Von Willebrand

VWF

VWF Activity

Specimen Required

Preferred Container/Tube: 3.2% Citrate Blue Top - 2.7 mL
Specimen Volume: 2.7 mL
Acceptable Container/Tube: 3.2% Citrate Blue Top - 1.8 mL
Specimen Volume: 1.8 mL
Platelet poor plasma (PPP):
Specimen Volume: 2 - 1 mL aliquots platelet poor plasma (PPP)
Specimen Minimum Volume: 1 - 1 mL aliquot platelet poor plasma (PPP)

Specimen Collection Information

Sample MUST be filled to indicated line on blue top tubes for proper blood:anticoagulant ratio (9:1). Do not fill past the bottom of the cap to avoid rejection for being too full (>9:1 ratio).

 

For Outreach, other than OSU Patient Service Centers: Samples must be processed and received frozen/must remain frozen in transit. 

 

For sending frozen plasma specimens, process immediately upon receipt. Centrifuge capped specimen tube in accordance with CLSI H21, at a speed and time required to consistently produce platelet-poor plasma (PPP = platelet count <10 K/uL). Transfer PPP into plastic aliquot tubes, cap and freeze. DO NOT transport until specimens are completely frozen. 

Specimen Stability Information

Ambient: 
     Whole blood: 4 hours
      Platelet poor plasma: 4 hours
Refrigerated: 
Frozen: 
      Platelet poor plasma: PPP removed from the cells and frozen at -20°C or ideally -70°C.

Rejected Due To

  • Ambient WB specimens >4 hours old
  • Ambient PPP specimens >4 hours old
  • Clotted specimens
  • Underfilled or overfilled tube
  • Gross hemolysis
  • Gross icterus

Reference Values

50-203 % Activity

Method Description

Latex turbidimetric immunoassay (Siemens Innovance® vWF Ac)

Performing Lab

Clinical Lab UH

Day(s) Performed

Monday through Friday (excludes OSU holidays)

Report Available

Same day/1 day

Reporting Name

Von Willebrand Factor GP1bM Activity

CPT Code Information

85397