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Test Code ORGANOXBLDGAS Blood Gas, Perfusate

Clinical Information

This panel is intended only for testing perfusate from isolated ex vivo organs intended for transplantation. It is not intended for whole-blood specimens, other patient or donor specimens, or donor-suitability testing. Testing of organ perfusate for this purpose is not subject to CLIA under CMS memorandum QSO-25-18-CLIA.

Synonym

Perfusate, OrganOx

Profile Information

Reporting Name Available Separately Always Reported
PO2 No Yes
GLUC No

Yes

LACT Yes Yes
PH No Yes
PCO2 No Yes
K Yes Yes
HCO3 No Yes
BGHGB No Yes

 

Specimen Required

Preferred Container/Tube: Dry Lithium Heparin Syringe

Acceptable Container/Tube: Dry Lithium Heparin Syringe
Specimen Volume: Full syringe

Specimen Minimum Volume: Full syringe

Specimen Stability Information

Ambient: 30 minutes
Refrigerated: 30 minutes
Frozen: unacceptable

Rejected Due To

Unacceptable container type
Incorrect specimen source
Quantity not sufficient
Clotted specimens
Gross hemolysis
Frozen specimens
Received >30 minutes from collection time

Interpretation

Reference intervals and clinical decision limits have not been established for organ perfusate. Results should not be interpreted using whole-blood reference intervals and should be evaluated according to the organ type, perfusion solution and conditions, collection time point, and the transplant program’s established protocol.

Cautions

Contamination of samples with ambient air will drive the pO2 to equilibrate with ambient air (150 mmHg) leading to falsely low or falsely elevated results depending on the initial pO2 level; as well as falsely decrease the pCO2; and falsely increase the pH, which will also affect the portion of calcium bound to protein. Therefore, care should be taken to expel all air bubbles from all blood gas samples.

Syringes that are not completely filled will have increased heparin concentrations that can lead to erroneous measurements of pO2, pCO2, sodium, potassium, and ionized calcium.

Potassium results are elevated with in vitro hemolysis. Hemolysis may be undetected in certain whole blood potassium measurements. Interpret results with caution.

Method Description

pO2, Glucose, Lactate: Amperometry

pH, pCO2, K: Potentiometry

HCO3: Log (HCO₃ (c)) =   pH + log(pCO₂) – 7.608 mmol/L

tHgb: Oximetry

Performing Lab

Clinical Lab UH

Day(s) Performed

Monday through Sunday

Report Available

Same day/1 day

Reporting Name

Blood Gas, Perfusate