Specimen Collection Information

DO NOT FILL TUBES WITH A SYRINGE. Tubes should be at 17-25C (63-77F) at the time of blood filling. Collect 1 ml of blood into each of the 4 tubes.

Order of draw:

1. Gray (Nil)
2. Green (TB1 )
3. Yellow (TB2)
4. Purple (Mitogen)

Tubes fill slowly. Keep each tube on the needle for 2-3 seconds after tube appears to have completed filling. When tube is upright, blood level must be within the small black mark on the label on all 4 tubes. Over filling or under filling will cause the test to be rejected. Immediately shake the tubes 10 times, just firmly enough to ensure inner surface of tube is coated in blood (to solubilize antigens on tube walls). Do not vigorously shake them. Over-energetic shaking could lead to aberrant results. The tubes can be labeled with any of the 4 QFT test labels. Make sure the QuantiFERON band and entire specimen volume is visible. WRITE THE COLLECT TIME ON ALL THE TUBES. Maintain the tubes at room temperature.